| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.035932 |
| Chromosome: | chromosome 7 |
| Location: | 4920927 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g346672 | (1 of 3) IPR000104//IPR006553 - Antifreeze protein, type I // Leucine-rich repeat, cysteine-containing subtype | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCATTCCGCTGCTGCCATCAGATGCTGCCGCCTCCATCTCCTCCTCCTCG |
| Internal bar code: | TCGGATTACATCTGCTTAATCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2344 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGTGGTGAGAGTTGGGTAA |
| Suggested primer 2: | CGGCTTGCACGAACTTCTTC |