| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.035939 |
| Chromosome: | chromosome 11 |
| Location: | 858798 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467648 | RBL3 | Rhomboid-like protease; (1 of 4) PTHR22936//PTHR22936:SF32 - RHOMBOID-RELATED // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCCCGATTTGGGTCTGTGTGGGACCGTCCGCGGCCTCGAGTGCGGAGT |
| Internal bar code: | GTGCGTCTCAGGGGCGGCCCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2151 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 60 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 60 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGAGCGACTGTATACGTGA |
| Suggested primer 2: | AGAGCATACGTTACGGAGCG |