Insertion junction: CLIP2.035940_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.035940
Chromosome:chromosome 12
Location:2160826
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g509500 (1 of 1) IPR000595//IPR002048//IPR011992//IPR018490//IPR029530 - Cyclic nucleotide-binding domain // EF-hand domain // EF-hand domain pair // Cyclic nucleotide-binding-like // Cell division control protein 31 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGTCATTTGCGCACCATGTGCAACCGTGGCCGGTGCTAGCGCCTCAACAC
Internal bar code:ACATTTCCACACCAATCGATCG

Confirmation - LEAP-Seq

LEAP-Seq distance:1624
LEAP-Seq percent confirming:69.697
LEAP-Seq n confirming:23
LEAP-Seq n nonconfirming:10
LEAP-Seq n unique pos:33

Suggested primers for confirmation by PCR

Suggested primer 1:AGCTTATTGGAGATCCGGCG
Suggested primer 2:GTATGTCGCGCTCTCTCGAA