Insertion junction: CLIP2.035945_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.035945
Chromosome:chromosome 9
Location:1078232
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g401900 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AAGGTGGGAGAGCCGTTGGTGGAAGTCGGCCGCCCCGCCATTATCACGGG
Internal bar code:CTATCGCAAAAAATCGGTTCGT

Confirmation - LEAP-Seq

LEAP-Seq distance:407
LEAP-Seq percent confirming:70.0
LEAP-Seq n confirming:14
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:20

Suggested primers for confirmation by PCR

Suggested primer 1:CGCATCTCTACTCGCCATGT
Suggested primer 2:CACGCAAGAGCAACCTGTTC