| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.035961 |
| Chromosome: | chromosome 3 |
| Location: | 5358503 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g183300 | (1 of 4) 2.7.9.5 - Phosphoglucan, water dikinase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGCCAACTGTGATGCTGAAGAAGCCTTCTCCCGCTCGTGAGTTTGCAG |
| Internal bar code: | TATGCTTATCCTTCCGGGGGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2717 |
| LEAP-Seq percent confirming: | 96.875 |
| LEAP-Seq n confirming: | 31 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTCAGCACCTACCCGTTGG |
| Suggested primer 2: | CCTGCCATGCACCAGACTTA |