| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.035963 |
| Chromosome: | chromosome 3 |
| Location: | 8757891 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g207825 | (1 of 1) K03241 - translation initiation factor eIF-2B subunit gamma (EIF2B3) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCGGGCCGTGCCGGTGTCAAACCCACGCGCGAACCCCCAGCGCGCCCGC |
| Internal bar code: | GCTAGGTTTCGTTGGTGACGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1475 |
| LEAP-Seq percent confirming: | 95.5556 |
| LEAP-Seq n confirming: | 43 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 45 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTCGGGTGTTCTGGTGACC |
| Suggested primer 2: | CTACCGTAACCCCTTGCGAC |