Insertion junction: CLIP2.035977_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.035977
Chromosome:chromosome 11
Location:2469440
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g095114 (1 of 228) IPR029063 - S-adenosyl-L-methionine-dependent methyltransferase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATCTCTCCGCATCTACCTACTGTCGAGTGCGGCTCCGTTGTCAAAGCGTG
Internal bar code:TGGGTCAGTTTGTAATCGGGAA

Confirmation - LEAP-Seq

LEAP-Seq distance:449
LEAP-Seq percent confirming:85.7143
LEAP-Seq n confirming:6
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:AACAGCAGGAATGTAGGGGC
Suggested primer 2:GGCGCTGTTACGTTTGTGTT