| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.035984 |
| Chromosome: | chromosome 6 |
| Location: | 7085897 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g297800 | (1 of 3) IPR013694 - VIT domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTTGTGTATAGAGAACATAAATTAGTGGTGTCTCAGGCGAAGGGCGCT |
| Internal bar code: | ATAAATTCACGTTTTTATGATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1668 |
| LEAP-Seq percent confirming: | 70.9677 |
| LEAP-Seq n confirming: | 22 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGATGCCATGTCTAGTCGGG |
| Suggested primer 2: | GGCCCGTTTTCGAAGAACTG |