Insertion junction: CLIP2.035991_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.035991
Chromosome:chromosome 10
Location:2908861
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g439800 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGCTGTTGTCACCATCAGTTGCGTCGACCGTCTGCAGGGCTGGCAGGTCC
Internal bar code:TGCTGCGACCGTGAGGTGCCCG

Confirmation - LEAP-Seq

LEAP-Seq distance:779
LEAP-Seq percent confirming:60.0
LEAP-Seq n confirming:9
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:15

Suggested primers for confirmation by PCR

Suggested primer 1:TGCCGCTGCTCCTGATAAAA
Suggested primer 2:AAATCCACTCCAACGCTCGT