| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.035997 |
| Chromosome: | chromosome 7 |
| Location: | 671538 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g317250 | CHI5 | (1 of 2) K01183 - chitinase (E3.2.1.14); Chitinase-like hydrolase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGGGAAGAGCGCGCTCTAGCCAGCTTTTTGTCGGGCCTGCTGCCTGCCT |
| Internal bar code: | GTTTGACCTCCTTCTAACCAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 451 |
| LEAP-Seq percent confirming: | 10.3448 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGTACAGTCCCGTACATCG |
| Suggested primer 2: | GTGCACCACCTACATGAGCT |