| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.036028 |
| Chromosome: | chromosome 14 |
| Location: | 1170041 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g615350 | THB2 | Truncated hemoglobin; (1 of 2) IPR016339 - Truncated hemoglobin, group 1 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGACCTATGCTTTCGGTGGCGCCACGGGATACACGGGCCGCGACCTGGCC |
| Internal bar code: | TTTAATCGATTGGCGGTGACTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1688 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCCATATGTCCAGCCCTCA |
| Suggested primer 2: | CACCCTTTCCCGTTTCCGTA |