| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.036034 |
| Chromosome: | chromosome 3 |
| Location: | 8614246 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g210513 | (1 of 2) K05894 - 12-oxophytodienoic acid reductase (OPR) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTTCCAAGACTCCAGCTGCTCAGGCGTGTACAGGCCGGGGGTGTTCAGG |
| Internal bar code: | CTACCGTCACAAGCTGTAACCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3754 |
| LEAP-Seq percent confirming: | 91.6667 |
| LEAP-Seq n confirming: | 88 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 96 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCCTCGTCAGTGAAACGTC |
| Suggested primer 2: | CCTTGACGATGCGCTTGATG |