| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.036047 |
| Chromosome: | chromosome 7 |
| Location: | 487524 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g315650 | SMM23 | (1 of 1) 2.1.1.164 - Demethylrebeccamycin-D-glucose O-methyltransferase; S-adenosyl-L-methionine-dependent methyltransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAGACGCGGGCTCCTTCGTTTCCCTTTACACGCACACACGCACATGGCA |
| Internal bar code: | GCATTAATTTGAAGGTCCCGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 901 |
| LEAP-Seq percent confirming: | 77.7778 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTGCTGTTGCTGTCTGGTG |
| Suggested primer 2: | GAGCTATGTTTCGCGCCTTG |