| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.036048 |
| Chromosome: | chromosome 6 |
| Location: | 7869701 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g303650 | (1 of 1) K15027 - translation initiation factor 2D (EIF2D) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCTGCTGGCTTTGTAGGGCCTAGTAGCGGGGTGTGTGCGGGAGAATGCG |
| Internal bar code: | GGTTACATTTAATTTCGTGCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1365 |
| LEAP-Seq percent confirming: | 83.3333 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTGTGCTGTGGAACCAACA |
| Suggested primer 2: | GCGGTTGAAGCAGAAGCAAA |