| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.036058 |
| Chromosome: | chromosome 16 |
| Location: | 3001795 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g663850 | SSS5 | Soluble starch synthase V; (1 of 2) PTHR12526//PTHR12526:SF304 - GLYCOSYLTRANSFERASE // STARCH SYNTHASE 4, CHLOROPLASTIC/AMYLOPLASTIC-RELATED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCAATGGGATCAGGGCCAAAACACTCGCCGTACTTCCCTGGTGTGACTG |
| Internal bar code: | TTGGTTTGCCTAGATCCTTTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3023 |
| LEAP-Seq percent confirming: | 83.6364 |
| LEAP-Seq n confirming: | 92 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 110 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACGAACACCTGCACATTGC |
| Suggested primer 2: | CCAATGGCCATGACAGCAAC |