| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.036063 |
| Chromosome: | chromosome 3 |
| Location: | 7340528 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g206650 | (1 of 1) PTHR24349:SF88 - SERINE/THREONINE-PROTEIN KINASE CG17528 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTATCACCTGGCGCCGGAGGCAATCAGCGGCTCCGGCTACGGCCGGCCA |
| Internal bar code: | CCGGTGGGGGTTTATGTACGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2629 |
| LEAP-Seq percent confirming: | 55.7143 |
| LEAP-Seq n confirming: | 39 |
| LEAP-Seq n nonconfirming: | 31 |
| LEAP-Seq n unique pos: | 70 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATAGGCACCCACGCTCTAC |
| Suggested primer 2: | ACACCGCATTCAACTTTGGC |