| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.036084 |
| Chromosome: | chromosome 3 |
| Location: | 5657786 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g186400 | SCR1 | (1 of 3) PF03803 - Scramblase (Scramblase); Putative phospholipid scramblase | CDS |
| Cre03.g186450 | LNM1 | Lysine N-methylase; (1 of 5) PTHR13271//PTHR13271:SF28 - UNCHARACTERIZED PUTATIVE METHYLTRANSFERASE // SUBFAMILY NOT NAMED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCAGATGATGCCCGGCAACCTGGAGGCGCTGGTGAGTTGCAAATGCCAA |
| Internal bar code: | TTCATCGTATCAATGATCCCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3938 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 6 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTACGTACGGAGGTGCTACG |
| Suggested primer 2: | CACGTTGGTTTGGCTTGAGG |