| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.036140 |
| Chromosome: | chromosome 16 |
| Location: | 7064855 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g674852 | HAP2,GCS1 | (1 of 1) PF10699 - Male gamete fusion factor (HAP2-GCS1); Generative cell specific-1 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCCTTTAACTGGAAACCCCTGGAAGTCATCCTGCGCCCCAGCAACAAAG |
| Internal bar code: | TGCAACGGGTTACGTGGTGTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1146 |
| LEAP-Seq percent confirming: | 96.0 |
| LEAP-Seq n confirming: | 24 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTTGCGGCCAATCAGTATG |
| Suggested primer 2: | CTGGACACCGAGACGTGAAA |