Insertion junction: CLIP2.036193_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.036193
Chromosome:chromosome 3
Location:6128575
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g800355 (1 of 3) PF03732//PF13650 - Retrotransposon gag protein (Retrotrans_gag) // Aspartyl protease (Asp_protease_2) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCCAGAGCAACTGACGCCGACTTGCGCAGGCCGCGGACGTAGGCTCGCA
Internal bar code:CAATCTATAATTGAGGATGAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:5203
LEAP-Seq percent confirming:24.4898
LEAP-Seq n confirming:24
LEAP-Seq n nonconfirming:74
LEAP-Seq n unique pos:98

Suggested primers for confirmation by PCR

Suggested primer 1:TGGTGTTGTGTGTGACAGCT
Suggested primer 2:CACATCATGTTCAGCTCGCG