Insertion junction: CLIP2.036196_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.036196
Chromosome:chromosome 2
Location:6770450
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g387838 (1 of 3) PF03647 - Transmembrane proteins 14C (Tmemb_14) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGGTGGCCCCGTACGCCGCACCAAAAGCCAGGCCACCAAGCAGCTGCGCG
Internal bar code:TTCGCATTTCGGGCGGGGTCAA

Confirmation - LEAP-Seq

LEAP-Seq distance:2383
LEAP-Seq percent confirming:96.4912
LEAP-Seq n confirming:55
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:57

Suggested primers for confirmation by PCR

Suggested primer 1:ACGTTTCTGTTTGTCGCTGC
Suggested primer 2:CACTGCCATTTCCCTCCCTT