Insertion junction: CLIP2.036201_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.036201
Chromosome:chromosome 17
Location:5270749
Confidence (%):63
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACAGGCTGCTTGGGGAGCTGTATTGCCCAGCAACTCGGGCCAGACGGAG
Internal bar code:GTAATGTCTACGGAATCCATCA

Confirmation - LEAP-Seq

LEAP-Seq distance:1905
LEAP-Seq percent confirming:33.3333
LEAP-Seq n confirming:4
LEAP-Seq n nonconfirming:8
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:TCCCTCTCTCTTCAGTCCCG
Suggested primer 2:GTGCTGTGATGCCCTACCTT