| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.036204 |
| Chromosome: | chromosome 5 |
| Location: | 1144082 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g248401 | (1 of 4) 3.4.21.19 - Glutamyl endopeptidase / V8 proteinase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCTAGCAAAGGAGCGGGCGCCGTCAACGAACCCAGCACCTTGCTTGGCA |
| Internal bar code: | AATATCCGAAGCACACGACGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3375 |
| LEAP-Seq percent confirming: | 95.0 |
| LEAP-Seq n confirming: | 38 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAGCTTACAGGGCATGCAT |
| Suggested primer 2: | TCACCGTCTGTTGACAGCTC |