Insertion junction: CLIP2.036229_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.036229
Chromosome:chromosome 14
Location:2766786
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre14.g626400 CYP33,CYP743C1 (1 of 3) 1.14.14.1//5.3.99.5 - Unspecific monooxygenase / Xenobiotic monooxygenase // Thromboxane-A synthase / Thromboxane synthetase; Cytochrome P450, CYP197 superfamily intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATGACCAACAACACACCGAGACCAAAACCAGCCCACACAGAGTGCGACGG
Internal bar code:AAACATGACGGTGCGGGACACG

Confirmation - LEAP-Seq

LEAP-Seq distance:162
LEAP-Seq percent confirming:34.4828
LEAP-Seq n confirming:10
LEAP-Seq n nonconfirming:19
LEAP-Seq n unique pos:29

Suggested primers for confirmation by PCR

Suggested primer 1:CTGTGTCTGCGAGTCTGTGT
Suggested primer 2:GAGGTCTGGAGGAGTCGACT