| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.036252 |
| Chromosome: | chromosome 4 |
| Location: | 3085870 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| intergenic |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTTGCTTATCCCTGCACCTGGTCTACCTGACCTGCGTGTGACCAAGTC |
| Internal bar code: | GAATTGTGATATGTCTGCTGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3662 |
| LEAP-Seq percent confirming: | 96.5517 |
| LEAP-Seq n confirming: | 84 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 87 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGTCTAGGCGGGTTGGATA |
| Suggested primer 2: | GTCAAGAGTACCCCAACCCG |