| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.036252 |
| Chromosome: | chromosome 12 |
| Location: | 2467336 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g507450 | SYP4 | Trans-Golgi network Qa-SNARE protein; (1 of 1) K08489 - syntaxin 16 (STX16) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCCTCCCCGCCCTCCGCGCACTCACTTGGTCCCCTCGTCCGCCTCCACA |
| Internal bar code: | TTTTATTGTATTATTGATGTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 149 |
| LEAP-Seq percent confirming: | 33.3333 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGTGTGGGTTTGTGGGTGT |
| Suggested primer 2: | TGAGCGCGATGATGCAAATG |