| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.036254 |
| Chromosome: | chromosome 10 |
| Location: | 966123 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g424600 | (1 of 1) PF14771 - Domain of unknown function (DUF4476) (DUF4476) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGGCGCTCGTACATGCGGTCCAGGCCGGCCTGGCAGGCAGGCGGGCGCG |
| Internal bar code: | AGTAGTTGATATGTAACGAGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 178 |
| LEAP-Seq percent confirming: | 7.14286 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACGCCTCCCACTCATTCAA |
| Suggested primer 2: | GGAGGTTTGCGTGAGAGGAA |