| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.036257 |
| Chromosome: | chromosome 2 |
| Location: | 911292 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g079000 | POB21 | Proteome of basal body 21 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCTGGTTGTATGCGCGACTATGGCAATAGGGAGCTTTGTGCCGGGGACG |
| Internal bar code: | TTGAGTCAGCACTCAGGTATAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 548 |
| LEAP-Seq percent confirming: | 80.0 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCACCATCTGCCTGTAGGTT |
| Suggested primer 2: | TCAGGACTACCGGCACTACA |