Insertion junction: CLIP2.036260_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.036260
Chromosome:chromosome 17
Location:211873
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g697300 CEP16 Cysteine endopeptidase; (1 of 1) 2.7.11.1//3.4.22.67 - Non-specific serine/threonine protein kinase / Threonine-specific protein kinase // Zingipain / Zingibain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CATATGGTGGGCCTGCGACCCCACCGACGCCCGGCAGTGGGTGGAGCTGC
Internal bar code:AATCACCTAGGCTTCGTCATCC

Confirmation - LEAP-Seq

LEAP-Seq distance:3689
LEAP-Seq percent confirming:96.4602
LEAP-Seq n confirming:109
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:113

Suggested primers for confirmation by PCR

Suggested primer 1:AGCCAAACTGCACTCTCCTC
Suggested primer 2:CACCCATGCACACAAACAGG