| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.036296 |
| Chromosome: | chromosome 11 |
| Location: | 624875 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467611 | (1 of 10) PF01062 - Bestrophin, RFP-TM, chloride channel (Bestrophin) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCCAGACCCCAAACTCCAAACTCCATGGTATGATGGGCAAACTCCATGG |
| Internal bar code: | GCTAATTCCTTTCCGCCTCATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 330 |
| LEAP-Seq percent confirming: | 90.0 |
| LEAP-Seq n confirming: | 9 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATGCACCATTCGCGTTCTT |
| Suggested primer 2: | ACACATAGACAGGTGTCGCG |