Insertion junction: CLIP2.036321_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.036321
Chromosome:chromosome 17
Location:4321336
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g730100 RSE1,RSEP1 (1 of 3) 3.4.24.85 - S2P endopeptidase / Sterol-regulatory element-binding proteins intramembrane protease; Intramembrane metalloprotease intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCAATTGAGGCCCCTCCGTGTATTGAAGCTGACATCCATTCGACGCGGC
Internal bar code:GGCGACGAAAGTGCGAAGTCAA

Confirmation - LEAP-Seq

LEAP-Seq distance:2525
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:48
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:48

Suggested primers for confirmation by PCR

Suggested primer 1:TCTCGGCAAAGGGGTACAAC
Suggested primer 2:CCGAGATGGAGTTCTACGGC