| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.036324 |
| Chromosome: | chromosome 2 |
| Location: | 7876348 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g145850 | FAP112 | Flagellar Associated Protein 112; (1 of 3) IPR006130 - Aspartate/ornithine carbamoyltransferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCCACTGCCACCAAGCGCTTTGTGGCGGGTCAGGCGGCGGCGGTGGCGG |
| Internal bar code: | CCTAGTCATAGGAATATGGCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1455 |
| LEAP-Seq percent confirming: | 2.22222 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 44 |
| LEAP-Seq n unique pos: | 45 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGCCTAGTTGTGCACGGAT |
| Suggested primer 2: | TTCCCCTTCTGCCGTCTTTC |