| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.036348 |
| Chromosome: | chromosome 2 |
| Location: | 1790021 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g086750 | (1 of 1) PTHR12709:SF5 - DNA-DIRECTED RNA POLYMERASE I SUBUNIT RPA43 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGCTGGTGGATGCTCCCCGACTTCTCCTCCGCCGGCCTGCCACACGGCC |
| Internal bar code: | TTCATCCCGCCACGCGGAAGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2054 |
| LEAP-Seq percent confirming: | 80.0 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTACTCTCCCTGCCCTTCCT |
| Suggested primer 2: | GCGGCAATCAGTAGCAATGG |