| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.036358 |
| Chromosome: | chromosome 2 |
| Location: | 6680107 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g387134 | (1 of 3) PF04379 - Protein of unknown function (DUF525) (DUF525) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAAGTTGCAAGTTCCAAGTCATCCCTCGCTGACCTGGTATACGAGTCCTG |
| Internal bar code: | ACTATAGGTCGACTGTACAACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1676 |
| LEAP-Seq percent confirming: | 94.0 |
| LEAP-Seq n confirming: | 47 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 50 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAACGAGCATTAGAGCGTG |
| Suggested primer 2: | AAGGAGCCTACGAGGTGCTA |