Insertion junction: CLIP2.036359_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.036359
Chromosome:chromosome 6
Location:5462434
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g286050 MCP20 Mitochondrial substrate carrier protein; (1 of 1) K15118 - solute carrier family 25, member 38 (SLC25A38) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCACGGCGGCGGGATGCGCTGCATGTGGACCGAGGGGCCGAGGGGCGTC
Internal bar code:GCGCTTCTTAGACGCTCTCACT

Confirmation - LEAP-Seq

LEAP-Seq distance:454
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:14
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:AGGTGGCAAAACAGGGAACA
Suggested primer 2:TATCCTATCCGCAACGCCAC