| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.036403 |
| Chromosome: | chromosome 3 |
| Location: | 3456615 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g166950 | PGM5 | (1 of 1) K01834 - 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (PGAM, gpmA); Phosphoglycerate mutase | CDS/intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGCACATCTGGCGCCGCAGCTACGACACACGTCCGCCGCCCCTGGAGCC |
| Internal bar code: | CTGCTTTTATTTTCCTGCAGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 587 |
| LEAP-Seq percent confirming: | 43.75 |
| LEAP-Seq n confirming: | 14 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTCCCCCTTACCTTGAGGC |
| Suggested primer 2: | GCTCCCTACACTCAACCCAC |