Insertion junction: CLIP2.036405_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.036405
Chromosome:chromosome 8
Location:3426933
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre08.g378250 SMM32 (1 of 1) 2.1.1.85 - Protein-histidine N-methyltransferase / Protein methylase IV; S-adenosyl-L-methionine-dependent methyltransferase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCTCCGCTGCCCCCACCCCACCTCACCCTCCGCTGCCCCCACCCCACCC
Internal bar code:ATGGTGTGTTCACCAATACAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:60
LEAP-Seq percent confirming:4.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:24
LEAP-Seq n unique pos:25

Suggested primers for confirmation by PCR

Suggested primer 1:CCAAAACACAGGTTGGCGAG
Suggested primer 2:AGGAGGGGGCTTACACTCAT