Insertion junction: CLIP2.036405_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.036405
Chromosome:chromosome 8
Location:3426941
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre08.g378250 SMM32 (1 of 1) 2.1.1.85 - Protein-histidine N-methyltransferase / Protein methylase IV; S-adenosyl-L-methionine-dependent methyltransferase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GATACCGGTACCAGCCCAAATTAAACCGAACCCGATGCAAAAGAGCTAGG
Internal bar code:ATGGTGTGTTCACCAATACAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:254
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:AGGAGGGGGCTTACACTCAT
Suggested primer 2:CCAAAACACAGGTTGGCGAG