| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.036424 |
| Chromosome: | chromosome 17 |
| Location: | 5148524 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g736650 | (1 of 2) K01256 - aminopeptidase N (pepN) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACAACTCACCCTCGGGCAGGCCGCTTAGCGTCAGGGTCTTGTCCGTCAGC |
| Internal bar code: | AATGTACCGTAGGGGAATTTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2080 |
| LEAP-Seq percent confirming: | 94.4444 |
| LEAP-Seq n confirming: | 17 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCTTCACATCGCCCCTTCC |
| Suggested primer 2: | GGAAGTAGGTAATCCCGCGG |