Insertion junction: CLIP2.036454_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.036454
Chromosome:chromosome 7
Location:3482759
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g335900 CPZ (1 of 1) 3.4.17.3 - Lysine carboxypeptidase / Lysine(arginine) carboxypeptidase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATATCGGTGTGTCTTTCTCGCAAGAGCGCGTGTGCAGGCGCTCTGCACGC
Internal bar code:CCTAGTCGAAACATCGTCGAGT

Confirmation - LEAP-Seq

LEAP-Seq distance:462
LEAP-Seq percent confirming:12.5
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:TACGCTTGGGTGTGCTGTAG
Suggested primer 2:GCAATCTCCAAATCGGACGC