| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.036458 |
| Chromosome: | chromosome 12 |
| Location: | 3223249 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g499800 | ASA10 | Mitochondrial F1F0 ATP synthase associated protein 10 | CDS |
| lncRNA_TCONS_00076407 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCTTGGGGCTGCGAGTGGGATGCCGGTCAGGCGACTGCAGATACACCAG |
| Internal bar code: | GGTCATTGCTCTATGCGTGTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2996 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 44 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 44 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCGTAGAGTCGAGAAGCAT |
| Suggested primer 2: | CCGAACCATAGTAGCCGCAT |