| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.036493 |
| Chromosome: | chromosome 3 |
| Location: | 5028565 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g179901 | mL116,OPR131,MRPL116 | mitochondrial ribosome protein mL116, Chlamydomonas-specific | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTAAAGCTGCCAAAGCTGCGGAGCTGAACATTGATTATTTCTAGCCGTAT |
| Internal bar code: | GATCGTGCGTGAAATGGGGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1065 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 16 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATCATGCGGTTGATGTGCC |
| Suggested primer 2: | CGAGGGGTCGTCAACATCAA |