Insertion junction: CLIP2.036500_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.036500
Chromosome:chromosome 17
Location:3143359
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g720700 ANK14 Predicted protein with ankyrin repeats; (1 of 7) PF12796//PF13637 - Ankyrin repeats (3 copies) (Ank_2) // Ankyrin repeats (many copies) (Ank_4) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGGCATGCAGCCTAAACACGACCCCTTGATTCGCACCAACCGTCCCCTT
Internal bar code:GTGTCATAGGTCTGACGGGTAC

Confirmation - LEAP-Seq

LEAP-Seq distance:80
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:9
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:TCTTCTTTACCCCAACGCCC
Suggested primer 2:CAGTCCCACCAGCTTGTCAT