Insertion junction: CLIP2.036509_3

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.036509
Chromosome:chromosome 17
Location:583157
Confidence (%):63
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCGTGCGCATTCATTTCCTTAAAAGCATCTTCGATAACAAATAGCAATTG
Internal bar code:GCACAAGCATCGACAATGCGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:897
LEAP-Seq percent confirming:20.6897
LEAP-Seq n confirming:6
LEAP-Seq n nonconfirming:23
LEAP-Seq n unique pos:29

Suggested primers for confirmation by PCR

Suggested primer 1:GCAAGCGGTTTGTGTGGAAT
Suggested primer 2:GAGGATCGCGACGATTGAGT