Insertion junction: CLIP2.036519_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.036519
Chromosome:chromosome 3
Location:6326517
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g192250 PHC69 (1 of 24) IPR003882//IPR024616 - Pistil-specific extensin-like protein // Pherophorin; Pherophorin-chlamydomonas homolog 69 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCACACCCACCGTCGCCGCCGTCCCCTGCGCCATCGCCGCCGTCCCCTG
Internal bar code:GTGTGGAAGGGGATGCGTTCGC

Confirmation - LEAP-Seq

LEAP-Seq distance:1326
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:7
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:CACAACATAGGCCACACCCT
Suggested primer 2:ATCTACGGCGCTCATGACTG