Insertion junction: CLIP2.036532_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.036532
Chromosome:chromosome 6
Location:7932492
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g304150 (1 of 4) PF06985//PF12796 - Heterokaryon incompatibility protein (HET) (HET) // Ankyrin repeats (3 copies) (Ank_2) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGTCGCTGGGGGTGCGTGTCGGGTGAGGGGGGGGAGGGGGCGACATGCG
Internal bar code:GGGTCTTGTACTACGCGAGTAT

Confirmation - LEAP-Seq

LEAP-Seq distance:3768
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:GGCATCTATTTGGCATGGCG
Suggested primer 2:TTGGATCACTTCTTCCGCCC