| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.036542 |
| Chromosome: | chromosome 12 |
| Location: | 4876478 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g523832 | (1 of 2) PTHR15885:SF1 - COILED-COIL DOMAIN-CONTAINING PROTEIN 174 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGCCGCTGCTCGGCTGCAACCATTGAGGCAGGACAGCAAGGGTTTAGGC |
| Internal bar code: | GTAGACAAACGTTGTTAAATCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2733 |
| LEAP-Seq percent confirming: | 77.2727 |
| LEAP-Seq n confirming: | 34 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 44 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCTTCTTGGCCACCTGATC |
| Suggested primer 2: | TGCCAGCCCTATCTACCCAT |