| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.036560 |
| Chromosome: | chromosome 1 |
| Location: | 809680 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g004500 | LEU1L,LEUL1 | (1 of 1) K01703 - 3-isopropylmalate/(R)-2-methylmalate dehydratase large subunit (leuC); Isopropylmalate dehydratase, large subunit | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCTGGGAAAATCCAGGGCTGGGGGGCCTGGAAGCCGCGGCCTGGGGCA |
| Internal bar code: | GGGGTTAGGGGTGCCATTGCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1182 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTGTTTGGAGGCCAGTACA |
| Suggested primer 2: | CAACGTGACCGAACACACAC |