Insertion junction: CLIP2.036566_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.036566
Chromosome:chromosome 12
Location:2213106
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g508900 MAPK6 (1 of 3) K04371 - mitogen-activated protein kinase 1/3 (MAPK1_3); Mitogen-Activated Protein Kinase 6 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGTTGTGCTGAGAATGGAGGGCAGTGTCAGAGTGGCATCCGGTGCACCAC
Internal bar code:TTTTTACCGGTAGAAGTAAGAC

Confirmation - LEAP-Seq

LEAP-Seq distance:4897
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:81
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:81

Suggested primers for confirmation by PCR

Suggested primer 1:ACAACGTCCATGCGTACCTT
Suggested primer 2:CAGCTGAGGTTTCTCCCCAG