| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.036576 |
| Chromosome: | chromosome 6 |
| Location: | 7822864 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g303350 | FAL9 | Conserved protein of unknown function | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACAGTCCAAGCACAGGTCCAACTCTTCCGCCACCAAGTTTATTGGCCCTC |
| Internal bar code: | AAGTTGTTAGAACGATCGAGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1138 |
| LEAP-Seq percent confirming: | 60.0 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTGGCGCCAAAACAGTTTG |
| Suggested primer 2: | GTGTGTGGGTGAGGAAGGAG |