Insertion junction: CLIP2.036595_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.036595
Chromosome:chromosome 17
Location:3929171
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g802075 (1 of 38) IPR002048//IPR011992 - EF-hand domain // EF-hand domain pair intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCTGCATGTGCAAGCGCGGATGTCCCAGGGCGCGAGGCCACCAATTTCA
Internal bar code:AGACGAGAAGGATCGCTCTTTC

Confirmation - LEAP-Seq

LEAP-Seq distance:3141
LEAP-Seq percent confirming:97.1429
LEAP-Seq n confirming:68
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:70

Suggested primers for confirmation by PCR

Suggested primer 1:TAGGACGTTTCGCTGGCTTT
Suggested primer 2:ACATCGTCTGACTGGCAGTG